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cy5 labeling enzymatic solution  (Jena Bioscience)


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    Jena Bioscience cy5 labeling enzymatic solution
    ( A ) Schematic illustration of pH-regulated enzymatic incorporation of nucleotides to substrate DNA molecules on a glass ceiling with spatioselectivity. Localized pH control induces a spatioselective deprotection of DNA strands. These deprotected strands can then be enzymatically elongated with <t>Cy5-labeled</t> ddATP nucleotides in parallel. The ceiling height is about 14 μm. ( B ) After parallelized enzymatic incorporation of <t>Cy5-labeled</t> ddATP to spatioselectively deprotected sites, epifluorescence imaging shows that the Cy5 fluorescence pattern (right) matches the randomly selected current stimulation pattern (left) exactly, thus confirming the enzymatic incorporation of nucleotides at the spatioselectively deprotected sites. The pH map at the midpoint of the 80-s-long stimulation is shown in the middle.
    Cy5 Labeling Enzymatic Solution, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cy5+labeling+enzymatic+solution/Taq+Polymerase+%2F+Labeling+Buffer/pmc09328676-182-19-27
    Average 95 stars, based on 61 article reviews
    cy5 labeling enzymatic solution - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "CMOS electrochemical pH localizer-imager"

    Article Title: CMOS electrochemical pH localizer-imager

    Journal: Science Advances

    doi: 10.1126/sciadv.abm6815

    ( A ) Schematic illustration of pH-regulated enzymatic incorporation of nucleotides to substrate DNA molecules on a glass ceiling with spatioselectivity. Localized pH control induces a spatioselective deprotection of DNA strands. These deprotected strands can then be enzymatically elongated with Cy5-labeled ddATP nucleotides in parallel. The ceiling height is about 14 μm. ( B ) After parallelized enzymatic incorporation of Cy5-labeled ddATP to spatioselectively deprotected sites, epifluorescence imaging shows that the Cy5 fluorescence pattern (right) matches the randomly selected current stimulation pattern (left) exactly, thus confirming the enzymatic incorporation of nucleotides at the spatioselectively deprotected sites. The pH map at the midpoint of the 80-s-long stimulation is shown in the middle.
    Figure Legend Snippet: ( A ) Schematic illustration of pH-regulated enzymatic incorporation of nucleotides to substrate DNA molecules on a glass ceiling with spatioselectivity. Localized pH control induces a spatioselective deprotection of DNA strands. These deprotected strands can then be enzymatically elongated with Cy5-labeled ddATP nucleotides in parallel. The ceiling height is about 14 μm. ( B ) After parallelized enzymatic incorporation of Cy5-labeled ddATP to spatioselectively deprotected sites, epifluorescence imaging shows that the Cy5 fluorescence pattern (right) matches the randomly selected current stimulation pattern (left) exactly, thus confirming the enzymatic incorporation of nucleotides at the spatioselectively deprotected sites. The pH map at the midpoint of the 80-s-long stimulation is shown in the middle.

    Techniques Used: Labeling, Imaging, Fluorescence

    Related Articles

    Mutagenesis:

    Article Title: CMOS electrochemical pH localizer-imager
    Article Snippet: .. For elongation of the deprotected strands with Cy5-labeled ddATP, the glass ceiling was spotted for 5 min with a Cy5-labeling enzymatic solution, consisting of 50 μM ddATP-Cy5 (Jena Bioscience, Jena, Germany), 8 μM TdT enzyme (Mutant F6, DNA Script, Paris, France), 1 mM CoCl 2 , and 1× TdT buffer (DNA Script, Paris, France). ..



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    cy5 labeling enzymatic solution  (Jena Bioscience)
    95
    Jena Bioscience cy5 labeling enzymatic solution
    ( A ) Schematic illustration of pH-regulated enzymatic incorporation of nucleotides to substrate DNA molecules on a glass ceiling with spatioselectivity. Localized pH control induces a spatioselective deprotection of DNA strands. These deprotected strands can then be enzymatically elongated with <t>Cy5-labeled</t> ddATP nucleotides in parallel. The ceiling height is about 14 μm. ( B ) After parallelized enzymatic incorporation of <t>Cy5-labeled</t> ddATP to spatioselectively deprotected sites, epifluorescence imaging shows that the Cy5 fluorescence pattern (right) matches the randomly selected current stimulation pattern (left) exactly, thus confirming the enzymatic incorporation of nucleotides at the spatioselectively deprotected sites. The pH map at the midpoint of the 80-s-long stimulation is shown in the middle.
    Cy5 Labeling Enzymatic Solution, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cy5+labeling+enzymatic+solution/Taq+Polymerase+%2F+Labeling+Buffer/pmc09328676-182-19-27
    Average 95 stars, based on 1 article reviews
    cy5 labeling enzymatic solution - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Schematic illustration of pH-regulated enzymatic incorporation of nucleotides to substrate DNA molecules on a glass ceiling with spatioselectivity. Localized pH control induces a spatioselective deprotection of DNA strands. These deprotected strands can then be enzymatically elongated with Cy5-labeled ddATP nucleotides in parallel. The ceiling height is about 14 μm. ( B ) After parallelized enzymatic incorporation of Cy5-labeled ddATP to spatioselectively deprotected sites, epifluorescence imaging shows that the Cy5 fluorescence pattern (right) matches the randomly selected current stimulation pattern (left) exactly, thus confirming the enzymatic incorporation of nucleotides at the spatioselectively deprotected sites. The pH map at the midpoint of the 80-s-long stimulation is shown in the middle.

    Journal: Science Advances

    Article Title: CMOS electrochemical pH localizer-imager

    doi: 10.1126/sciadv.abm6815

    Figure Lengend Snippet: ( A ) Schematic illustration of pH-regulated enzymatic incorporation of nucleotides to substrate DNA molecules on a glass ceiling with spatioselectivity. Localized pH control induces a spatioselective deprotection of DNA strands. These deprotected strands can then be enzymatically elongated with Cy5-labeled ddATP nucleotides in parallel. The ceiling height is about 14 μm. ( B ) After parallelized enzymatic incorporation of Cy5-labeled ddATP to spatioselectively deprotected sites, epifluorescence imaging shows that the Cy5 fluorescence pattern (right) matches the randomly selected current stimulation pattern (left) exactly, thus confirming the enzymatic incorporation of nucleotides at the spatioselectively deprotected sites. The pH map at the midpoint of the 80-s-long stimulation is shown in the middle.

    Article Snippet: For elongation of the deprotected strands with Cy5-labeled ddATP, the glass ceiling was spotted for 5 min with a Cy5-labeling enzymatic solution, consisting of 50 μM ddATP-Cy5 (Jena Bioscience, Jena, Germany), 8 μM TdT enzyme (Mutant F6, DNA Script, Paris, France), 1 mM CoCl 2 , and 1× TdT buffer (DNA Script, Paris, France).

    Techniques: Labeling, Imaging, Fluorescence